Critical appraisal Module

The research article addresses one of the fields in biotechnology, cellulose synthase that has attracted immense interest in the recent past. Previous knowledge in plant elongation indicated that information is transmitted from organized cortical microtubules to the cellulose-synthesizing complex leading to deposition along cell walls. Past studies such as by Fisher and Cyr (1998) did not have clear information on the microtubule alignment process. Later studies have revealed that plants establish cellulose microfribril orientation through cortical microtubules. This has been established through observation of cortical microtubule and cellulose synthase (CESA) complexes simultaneously which show that CESA particles change according to changes in cortical microtubules. However, this process is highly generalized as in special circumstance the alignment of cellulose microfibrils may occur differently from microtubules. This study`s scope is therefore to report a more specific procedure that identifies CESA interactive protein 1 (CSI1) as mediating the interaction between microtubules and cellulose synthase.
To do this, the researcher adopted a methodology that relies in analyzing primary data from several experiments in which microtubule array was disorganized through exposure to a microtubule-disrupting herbicide, oryzalin. The CESA interactive protein 1 (CSI1) was assessed whether it aligns to specific marked microtubules in a transgenic line. The results showed a majority of 84 +-4%. The colocalization was also examined over a longer period and results showed a dynamic relationship. Additional experiments showed that association of CESA complexes and cortical microtubules is dependent on CSI1.
The study probes the commonly held knowledge on the interaction between microtubules and cellulose synthase. Although previous studies have suggested that linker proteins are involved in how cellulose synthases associate with microtubules, the study reveals CSI1 as the linker protein. The study has proved this linkage clearly by having controls that confirm the role of CSI1. This is confirmed by two observations that CESA distribution depends on the interaction between CSI1 and microtubules and (ii) that the loss of either CSI1 or cortical microtubules will lead to a similar effect on cellulose synthase.
This knowledge is very critical in plant biotechnology. The knowledge that CESA interactive protein 1 (CSI1) is the linker in the interaction between microtubules and cellulose synthase can be applied in various ways such as plant genetics, plant physiology and even formulation of herbicides. Most important, the study`s conclusions have resulted into further research in the field. One of the studies is by Baskin and Gu (2013) who explore how the knowledge of the involvement of CSI1 in cellulose synthases and microtubules contributes to study of intercepting information flowing out from different kinds of cells (both animals and plants) and to many kinds of matrix to understand how organisms draw parallel lines, even if they never meet.
The study`s main conclusion is the confirmation of the hypothesis that the linker proteins involved in how cellulose synthases associate with microtubules is CESA interactive protein 1 (CSI1). The study went ahead and demonstrated the involvement of CSI1 in various scenarios and environments. To explain the results better to the audience, the researchers have included graphical presentations of their results inform of charts and actual images from their experiments. These not simply how data is presented but also increases the confidence in the study by presenting actual images from the study which the audience can assess their validity. From my convictions, I believe that that study, despite failing to show comprehensively the actual methodology used for possible replication of the study, the conclusions is right. This is further supported by the fact that the study is widely cited by other scholarly studies on the subject.
I think the research should be organized in amore reader-friendly manner. For most learners, there is an expectation that such research reports should follow a specific format for easier following. For instance, I noted that the report does not acknowledge the earlier trials to identifier the linker protein between cellulose synthases and microtubules. This would allow the audience to understand whether the researchers sued a different approach or paradigm from the used in the pat in attempting to identify this linker. Furthermore, the report does not clearly indicate the hypotheses from the very beginning though in the later parts of the report, reference is made to “the hypothesis”.
Despite the noted issues in the format and arrangement, the paper presents a critical issue in the field of plant physiology. The research presents a very important breakthrough in science which opens up further research in cellular communication. It is noteworthy that the results presented by the study have not received any meaningful opposition in the research field which tends to imply that majority of researcher sin the field are convinced by the study and the conclusions made are true. However, there is need for further similar studies to ascertain the findings beyond reasonable doubt.
All in all, the study is a meaningful read. Though the complex coding of some names can make it hard for a larger audience to follow, the paper presents valuable research findings in the field of plant physiology.
References
Baskin, T & Y. Gu 2012, Making parallel lines meet: Transferring information from
microtubules to extracellular matrix, Cell Adhesion & Migration vol. 6, no. 5, pp.1 – 5
Fisher, D 1998, Extending the microtubule/microfibril paradigm, Plant Physiology vol. 116, no.
3, pp. 1043 – 1051.
Li, S, L Lei, C Somerville & Y Gu, 2012, Cellulose synthase interactive protein 1 (CSI1) links
microtubules and cellulose synthase complexes, PNAS, vol. 109, no. 1, pp. 185-190.